Palmitoyl Tripeptide-1 (Pal-GHK) 200mg

Palmitoyl Tripeptide-1 Peptide

Palmitoyl Tripeptide-1 is an endogenous copper peptide that occurs in the tripeptide glycyl-L-histidyl-L-lysine. It has two variants — GHK with or without Cu (Copper). GHK appears to have a strong affinity for copper (II), and it is synthesized from plasma, although it has been isolated in other areas. Endogenous GHK production is considered to decline over time. ^[1]

In cases of injury, GHK may be released from tissue cells. That may be because GHK is present in various proteins that get broken down via hydrolysis during injury. For example, studies suggest that GHK is present in the “alpha 2(I) chain of type I collagen”, which “suggests that the tripeptide might be liberated by proteases at the site of a wound and exert in situ healing.” ^[2] GHK peptide may also be released from the breakdown of another extracellular matrix-binding protein involved in regulating cell shape and proliferation, called SPARC (Secreted Protein, Acidic, and Rich in Cysteine). GHK is found in tissues undergoing remodeling, such as during the process of angiogenesis.^[3]

When GHK is released from the breakdown of proteins like collagen and SPARC, this signaling appears to trigger the fibroblasts to begin synthesizing new collagen and other structural proteins in the skin structure and connective tissues like elastin and glycosaminoglycan. Further, this copper-binding peptide appears to affect genes that control recuperative responses to injury and stress. Its functions appear to include the following: tissue remodeling, anti-inflammatory response, pain perception inhibition, nootropic, anti-cancer action, blood vessel growth, and nerve outgrowth.

Specifications

Molecular Formula: C₁₄H₂₄N₆O₄

Molecular Weight: 340.4 g/mol

Synonyms: NSC661251, NSC-661251

GHK and GHK-Cu

GHK has a sequence inherent in SPARC protein and collagen molecules. Copper, on the other hand, is a transitional element of utmost importance to organisms with a cell membrane, extending from microorganisms upwards. Sequel to its conversion from oxidized -Cu (II) to reduced -Cu (I), it is considered to be a vital cofactor in a series of biochemical reactions accompanied by electron transfer. The changes in copper oxidation state may be an advantage, as dozens of enzymes may adopt it in catalyzing critical biochemical reactions such as detoxification, blood clotting, cellular respiration, antioxidant defense, and connective tissue regeneration.^[2] Copper also appears to be vital in iron metabolism and embryonic development, potentially essential for most metabolic reactions that occur in fetal development, oxygenation, and other biological processes.

Research

GHK Peptide and Cancer Cells
Tumor suppressor genes or anti-oncogenes, growth regulatory-, and DNA repair genes appear to be essential in cancer cell suppression and apoptosis (cell death). Following research by Hong et al., the copper-binding peptide may be linked to wound healing and skin structure remodeling.^[4]

In one study, GHK, alongside another molecule, appeared to have reversed the oncogene expressions of research models. Subsequently, researchers suggested that “Gly-His-Lys and securinine [may] reverse the differential expressions of these genes significantly, suggesting that they have combinatorial [actions].”

GHK Peptide and Tissue Repair
The endogenously occurring GHK molecule is considered to play a vital role in the repair of damaged tissues. Endogenous copper peptides are similarly vital, possibly accelerating the production of elastin and collagen proteins. They appear to support the production of glycosaminoglycans and hyaluronic acid, which aid in moisture retention. The introduction of synthetically developed copper peptide appears to support blood flow to areas of focused follicle growth, encouraging hair follicle development.

According to Campbell et al., GHK appears to support the production of TGF beta and members of the same species that initiate the repair process. The copper peptide, with the aid of TGF beta, may reset the gene expression of fibroblasts in research models of COPD. Campbell et al. also “suggest the need for additional studies to examine the mechanisms by which TGF beta and GHK each reverse the gene-expression signature of emphysematous destruction and the [impact] of this reversal on disease progression.” ^[5]

GHK Peptide and DNA Repair
DNA damage begins to accumulate at the onset of cellular aging. The GHK peptide has been researched for its potential to reset the activity of DNA repair genes, diminishing physiological decline.^[6] Studies are ongoing.

GHK Peptide and Antioxidant Characteristics
GHK appears to initiate 14 antioxidant genes and may repress two pro-oxidant genes. Pickart et al. propose that GHK tripeptide may have supporting actions against oxidative stress and free radicals.^[7] For example, active radicals are a major factor contributing to epidermal tissue wrinkling, photoaging, and light-associated damage.

Research indicates that the peptide GHK might play a role in neutralizing harmful free radicals, which are by-products generated during lipid peroxidation—a process where lipids degrade, particularly when exposed to UV light.^[8] The specific radicals potentially affected include 4-hydroxynoneal, acrolein, and malondialdehyde, among others, all of which are believed to contribute to cellular damage.

GHK Peptide and Inflammation
The antioxidant properties of GHK might also extend to various models of inflammation. There is some indication that GHK might interfere with mechanisms of oxidative stress and inflammation, such as iron release from ferritin, a protein complex involved in storing iron, thereby potentially mitigating lipid peroxidation. Studies suggest that GHK might reduce the formation of iron complexes within damaged tissues, potentially lowering inflammation levels.^[9]

It is hypothesized that GHK binds to ferritin channels, possibly reducing iron release by as much as 87%, which may play a role in mitigating further oxidative damage and inflammation in compromised tissues. In animal models, GHK has been observed to potentially suppress lung tissue inflammation caused by lipopolysaccharides—a component of bacterial cell walls—by inhibiting the activation of the NF-κB and p38 MAPK signaling pathways, which are believed to promote inflammatory responses.^[10]

This action might also lead to a decrease in the production of pro-inflammatory cytokines, such as TNF-1 and IL-6. Further studies suggest that GHK might have an inhibitory action on oxidative stress in epithelial cells by possibly upregulating the expression of Nrf2, a key regulatory protein that controls the expression of antioxidant proteins.^[11] The antioxidant activity of GHK may be stronger than other natural peptides like carnosine and reduced glutathione. This is especially true when looking at its ability to fight hydroxyl radicals in cell cultures